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Determination du profil des flores bacteriennes des zones littorales. Application a l'evaluation de l'impact des rejets ArchiMer
Bunetel, L; Segui, A; Guerin, J; Lerman, I; Cormier, M.
The principle of this method is to evaluate, by counts exercised upon selective media, the relative importance of the different bacteria families in the flora of the coastal waters. It is important to separate the contaminant species and the commensal species in the marine environment. Ten culture media distributed into four categories (selective, non selective, saline and non saline) were examined by numeration. Counts were transferred on a diagram allowing to plot down a profile of bacterial flora of water sampled from a given geographical area. The profiles are arranged by a classification method. The sampling points with similar or adjoining profiles were assembled and marked on the chart. In this way, the authors obtained areas representing the impact...
Tipo: Text Palavras-chave: Bacteria; Coastal waters; Microbiological culture; Sewage disposal; Microbial contamination; Bacterial counters; Bacteria collecting devices.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1004.pdf
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Etude au microscope electronique a balayage des relations entre bacteries et matieres en suspension en milieu marin ArchiMer
Cormier, M; Bassoulet, P; Pommepuy, Monique.
Near outfall areas numerous bacteria appear associated to colloidal organic matter present in littoral sea water. Arrangements into colonies are shown. Surface structures are observed which allow bacteria's attachment each to other and to organic matter.
Tipo: Text Palavras-chave: Bacteria; Outfalls; Electron microscopy; Flocculation; Colloids; Adhesion; Suspended particulate matter.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-966.pdf
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Implantation et survie de Campylobacter jejuni/coli chez la moule Mytilus edulis ArchiMer
Minet, Jacques; Sauvager, F; Cormier, M.
Illnesses due to marine bivalvia consumption are mostly associated with viruses as causative agents. In more than the half of cases, this agent remains unidentifies, nevertheless. So far as bacteria are concerned, Salmonella are found firstly. Campylobacters represent a major cause of gastrointestinal disease, with Salmonella, among humans. Whether campylobacteriosis may be acquired from shellfishes remains unclear. We undertook to comparatively appreciate the survival of Campylobacter jejuni/coli and Salmonella typhimurium in seawater and contaminated mussels. Bacterial enumerations where performes using microbial cultures and specific (with immunofluorescence) direct viable counts (of substrate responsive bacteria). During laboratory contamination...
Tipo: Text Palavras-chave: Campylobacter coli; Campylobacter jejuni; Mytilus edulis; Bivalvia; Pathogenic bacteria; Seafood; Microbial contamination; Pollution effects; Bacteria; Sea water; Survival.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1606.pdf
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Molecular epidemiological survey of rotaviruses in sewage by reverse transcriptase seminested PCR and restriction fragment length polymorphism assay ArchiMer
Dubois, E; Le Guyader, Soizick; Haugarreau, Larissa; Kopecka, H; Cormier, M; Pommepuy, Monique.
Rotavirus double-stranded RNA was detected directly in sewage treatment plant samples over a 1-year period by reverse transcription followed by PCR amplification of the VP7 gene and Southern blot hybridization. The presence of naturally occurring rotaviruses was demonstrated in 42% of raw sewage samples and in 67% of treated effluent samples, Amplified viral sequences were analyzed bg restriction enzymes. Ten different restriction profiles were characterized, most of which were found in treated effluent samples. A mixture of restriction profiles was observed in 75% of contaminated effluent samples, The profiles were compared with those obtained from human rotavirus isolates involved in infections in children from the same area (six different profiles were...
Tipo: Text
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00336/44720/44851.pdf
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Rejets urbains et contamination fecale ArchiMer
Pommepuy, Monique; Dupray, Elisabeth; Guillaud, Jean-francois; Derrien, A; L'Yavanc, Jacky; Cormier, M.
The behaviour of fecal bacteria was investigated in a turbid estuary which is also a shellfishing area. Sediments are polluted by sewage by the way of settlement, fecal bacteria mixed with estuarine water being able to survive a very long time (T90 are about several days). By measuring the increase of salt tolerance of the strains grown in natural estuarine organic matter, it was demonstrated that Escherichia coli is able to support the salinity of coastal water. Moreover, because light penetration is obstructed by suspended matter, the solar bactericidal effect is very low. A close relationship between T90 and light intensity received by the bacteria is found. These mechanisms can explain the low die-off rate found in turbid areas.
Tipo: Text
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00267/37860/35941.pdf
Registros recuperados: 5
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